Species-Specific PCR Assays for Differentiating Heterodera filipjevi and H. avenae
نویسندگان
چکیده
منابع مشابه
Distinguishing Heterodera filipjevi and H. avenae using polymerase chain reaction-restriction fragment length polymorphism and cyst morphology.
The cereal cyst nematodes Heterodera filipjevi and H. avenae impede wheat production in the Pacific Northwest (PNW). Accurate identification of cyst nematode species and awareness of high population density in affected fields are essential for designing effective control measures. Morphological methods for differentiating these species are laborious. These species were differentiated using poly...
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a survey of cereal fields of khuzestan province during 2008-2011 revealed that cereal cyst nematodes (ccns) are widely distributed in this region. the ccns were present in 37 and 35% of the 200 samples collected from wheat and barley fields respectively. the species were identified as heterodera avenae type b and h. filipjevi the morphological and morphometric identifications of which were conf...
متن کاملMolecular Polymorphism and Morphometrics of Species of the Heterodera avenae Group in Syria and Turkey.
Molecular characterization of the three most common cereal cyst nematode species of the Heterodera avenae group (H. avenae, H. filipjevi, and H. latipons), originating from various locations in major cereal-cultivating areas in Syria and Turkey, showed distinct restriction fragment patterns of the ITS-rDNA following PCR amplification and RFLP digestion with four endonucleases (Hae III, Hinf I, ...
متن کاملDevelopment of species-specific PCR and PCR-restriction fragment length polymorphism assays for L.infantum/L.donovani discrimination.
Discrimination of Leishmania infantum and L. donovani, the members of the L. (L.) donovani complex, is important for diagnosis and epidemiological studies of visceral leishmaniasis (VL). We have developed two molecular tools including a restriction fragment length polymorphisms of amplified DNA (PCR-RFLP) and a PCR that are capable to discriminate L. donovani from L. infantum. Typing of the com...
متن کاملUse of Chitin for Controlling Heterodera avenae and Tylenchulus semipenetrans.
The nematicidal effect of chitin, relative to other pesticides, was evaluated against two plant-parasitic nematodes, Heterodera avenae and Tylenchulus semipenetrans. Wheat seedlings, grown in soils artificially or naturally infested with H. avenae, were treated with 0.4% (w/w) ClandoSan (CLA) prepared from crustacean chitin, aldicarb (Temik 15G), or ethylene dibromide (EDB 90EC). The CLA treatm...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 2013
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis-01-13-0064-re